出品公司: | Agilent |
---|---|
载体名称: | pBC SK(+) |
质粒类型: | 基本克隆载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 3400 bp |
5' 测序引物及序列: | M13 fwd |
3' 测序引物及序列: | M13 rev |
载体标签: | -- |
载体抗性: | 氯霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
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pBC SK(+)载体 pBCSK(+)质粒基本信息
pBC SK(+)质粒图谱 pBCSK(+)载体图谱和pBC SK(+)载体序列 pBCSK(+)质粒序列多克隆位点信息
pBC SK(+)质粒 pBCSK(+)载体简介
pBC SK(+)质粒序列 pBCSK(+)载体序列
LOCUS pBC SK(+) 3400 bp ds-DNA circular SYN 25-11-2013 DEFINITION Phagemid vector derived from pBluescript II SK(+), with a chloramphenicol resistance gene. The MCS is reversed relative to pBC KS(+). ACCESSION . VERSION . KEYWORDS pBC SK(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3400) AUTHORS Agilent Technologies TITLE Direct Submission JOURNAL Exported 2017/2/28 from SnapGene 2.3.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3400 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(241..816) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR TDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG FPRQALNRGLPLGFRFSALRHLDPKKLD" primer_bind 600..616 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 653..760 /note="MCS" /note="pBluescript multiple cloning site" promoter complement(773..791) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(812..828) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 836..852 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(860..890) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(1214..1802) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2022..2124 /note="cat promoter" /note="promoter of the E. coli cat gene" CDS 2125..2784 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg 721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt 781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcgaccgaa 1921 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat 1981 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt 2041 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttgtcgaga 2101 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 2161 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 2221 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 2281 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 2341 ttacgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 2401 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 2461 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 2521 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 2581 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 2641 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 2701 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 2761 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 2821 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat 2881 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt 2941 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc 3001 agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcctt tttttctgat 3061 caaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3301 tttatcaagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 3361 caaatagggg ttccgcgcac atttccccga aaagtgccac //
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pBC SK(+)质粒 pBCSK(+)载体应用举例
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